Brew Bottle:Bactifeed 16oz Brew Bottle includes 1 Treatment of Bactifeed OST that covers 5000 sq ft Small Refill Bottle treats up to 20,000 sq ft | 1 capful per 2,000 sq ftORLarge Refill Bottle treats up to 40,000 sq ft | 1 capful per 2,000 sq ftBactifeed OST. A specially formulated blend of beneficial bacillus bacteria & mycorrhizal fungi. Bactifeed OST comes in a dry powder. The Bacteria and Mycorrhizal Fungi are in a dormant state and activate when mixed with water.The process of brewing makes Bactifeed OST stand out above liquid bacteria products. Brewing allows the bacteria to activate, breakdown and release stimulants in the Bactifeed mix, and reproduce so that you have trillions of bacteria by the time you spray.Each Brew Bottle cap-full of powder will cover a 2500 sq ft area follow these steps to get best results1. Add water to our 16 oz Bactifeed Brew Bottle & shake well2. Let mixture sit for at least 8 hours so that the BACTERIA & MYCORRHIZA FUNGI colonies can become fully active3. Spray Desired area and water for about 2 minutes afterwards to drive the beneficial microbes deeper into the soil.You can use a hose end sprayer, a watering can, backpack sprayer, etc.*when using a hose end spray take out the filter and set it to the setting that gets the largest water droplets. Refill Pack:Refill Pack treats up to 20,000 sq ft/Small or 40,000 sq ft/Large. Refill the Brew Bottle with 1 capful of the Refill Bottle's lid per 2,000 sq ft, fill with water, shake, and let brew for 8+ hours before applying. For best results: Apply first 2-3 treatments 2 weeks apart. Then once a month for the first year.Result times vary depending on soil. Harsh soils may take longer. Many see results with-in their first 30 to 90 days.